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OBJECTIVE@#To explore the molecular basis for an A subtype of the ABO blood group.@*METHODS@#The forward and reverse typing of the ABO blood group were identified by gel card and test tube methods. The ABO gene of the patient was detected by PCR-sequence specific primer (PCR-SSP). Exons 1 to 7 of the ABO gene was amplified by PCR and sequenced. The ABO gene was also subjected to subclone sequencing for haplotype analysis.@*RESULTS@#The patient's red cells showed weak agglutination with anti-A but non-agglutination with anti-B. The patient's serum showed 1+ agglutination with A cells and 4+ agglutination with B cells. Based on above serological characteristics, the patient was defined as Aw subtype of the ABO blood group. Sequencing analysis showed that the patient was heterozygous for c.106G>T, c.188G>A, c.189C>T, c.220C>T, c.297A>G, c.467C>T, c.543G>C, c.646T>A, c.681G>A, c.771C>T, c.829G>A, in addition with a c.261G deletion. Combined with the result of subclone sequencing, the ABO genotype of the patient was determined as ABO*AW.33. new/O.01.02, which harbored c.467C>T and c.543G>C variants compared with ABO*A1.01 and c.543G>C variant compared with ABO*A1.02. The novel allele has been submitted to GenBank with an accession number of MK302122.@*CONCLUSION@#A novel allele of Aw33 subtype has been identified with its GTA transferase gene harboring c.467C>T and c.543G>C variants compared with A1.01.
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Humans , ABO Blood-Group System , Genetics , Alleles , Exons , Genetics , Genotype , PhenotypeABSTRACT
Objective@#To investigate the feasibility of a nurse-led exercise and cognitive behavioral intervention for medium-advanced hepatocellular carcinoma patients with cancer-related fatigue, sleep quality and depression outcomes.@*Methods@#A total of seventy-nine medium-advanced hepatocellular carcinoma patients were enrolled in this study, the patients were randomly assigned to intervention group and control group. The control group given conventional nursing intervention, intervention group received exercise and cognitive behavioral therapy. The baseline characteristics between two groups were compared, the fatigue outcome was measured by the Piper Fatigue Scale at before intervention, after a 2-week intervention, after a 6-week intervention. Besides, depression and sleep quality were measured using the self-rating depression scale and the Pittsburgh sleep quality index questionnaire, respectively.@*Results@#After 2-week and 6-week of intervention, the fatigue scores in the intervention group (4.14±0.40 and 3.91±0.37) were markedly higher than in the control group (4.94±0.38 and 5.03±0.44) (t=9.116,12.195, all P<0.05). Moreover, the intervention group (44.74±6.01) points and (41.30±7.43) points demonstrated lower scores of depressions compared to the control group (50.53±7.49) and (48.39±6.16) after 2-week and 6-week of intervention (t=3.771,4.630, all P<0.05). The intervention group (7.25±0.36) points and (6.29±0.96) points also showed lower scores of sleep quality when compared to the control group (8.86±0.57) points and (7.86±0.91) points after 2-week and 6-week of intervention (t=2.013, 2.189, all P<0.05).@*Conclusion@#Nurse-delivered exercise and cognitive behavioral intervention have benefits in helping medium-advanced hepatocellular carcinoma patients to decrease cancer-related fatigue, depressive symptoms, and improving their quality of sleep.
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Objective To investigate the feasibility of a nurse-led exercise and cognitive behavioral intervention for medium-advanced hepatocellular carcinoma patients with cancer-related fatigue, sleep quality and depression outcomes. Methods A total of seventy-nine medium-advanced hepatocellular carcinoma patients were enrolled in this study, the patients were randomly assigned to intervention group and control group. The control group given conventional nursing intervention, intervention group received exercise and cognitive behavioral therapy. The baseline characteristics between two groups were compared, the fatigue outcome was measured by the Piper Fatigue Scale at before intervention, after a 2-week intervention, after a 6-week intervention. Besides, depression and sleep quality were measured using the self-rating depression scale and the Pittsburgh sleep quality index questionnaire, respectively. Results After 2-week and 6-week of intervention, the fatigue scores in the intervention group (4.14±0.40 and 3.91±0.37) were markedly higher than in the control group (4.94±0.38 and 5.03±0.44) (t=9.116,12.195, all P<0.05). Moreover, the intervention group (44.74±6.01) points and (41.30±7.43) points demonstrated lower scores of depressions compared to the control group (50.53±7.49) and (48.39±6.16) after 2-week and 6-week of intervention (t=3.771,4.630, all P<0.05). The intervention group (7.25±0.36) points and (6.29±0.96) points also showed lower scores of sleep quality when compared to the control group (8.86±0.57) points and (7.86±0.91) points after 2-week and 6-week of intervention (t=2.013, 2.189, all P<0.05). Conclusion Nurse-delivered exercise and cognitive behavioral intervention have benefits in helping medium-advanced hepatocellular carcinoma patients to decrease cancer-related fatigue, depressive symptoms, and improving their quality of sleep.
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Objective:To explore efficacy of the rehabilitative strategy in hospitalized schizophrenics.Method:Sixty-four schizophrenic patients were randomly divided into the intervention group and the control group.Both groups received the anti-psychotics therapy, but the teamwork education,medication management skills training module, symptom management skills training module were only given to the intervention group for ten weeks. And a one-year follow-up was carried out after their discharge from hospital. All subjects were evaluated with the Positive and Negative Syndrome Scale (PANSS), Nurses` Observation Scale for Inpatient Evaluation-30 (NOSIE-30), Insight and the Attitude to Treatment Scale, Social Disability Screening Schedule (SDSS), Morning Side Scale. We also recorded the relapse rate, reemployment rate, and re-hospitalization rate. Result:The clinical outcome of the intervention group was significantly superior to the control group on overall improvement according to PANSS (31.17?3.13 vs 52.34?13.02,7.34?1.08 vs 12.36?4.28,7.76?2.13 vs 13.26?4.50,16.13?1.82 vs 32.17?9.82 ),increase of active factor score of NOSIE-30 (-18.3?3.2 vs 14.4?6.7),and the reduction of inactive factor score of NOSIE-30(9.4?6.2 vs -22.3?7.4).The relapse rate(9.4% vs 54.8%),re-hospitalization rate(3.2% vs 42%),reemployment rate(37.5% vs 9.6%)were also better in the intervention group.Conclusion:The effect of rehabilitative strategy for schizophrenic patients not only improves the symptom, but also their social function.
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P53 gene (exon7～8) mutatins and p53 proteins and HPV 6，11，16，18-DNA were examined in 49 cervical carcinoma by immunohistochemistry, polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) in order to investigate their role and mutual relation and clinical significance in the onco genesis of cervical carcinoma. The results showed that first, p53 proteins posit ive rate was 48.98％, and not outstandingly related to the differentiation and the invasive degree of cervical carcinoma(P＞0.05); the defects of P53 gene (exon7～8) were not found but P53 (exon7～8) mutations were detected in 7 of 49(14.29％) cervical carcinoma; then, HPV16-DNA positive rate was much higher than HPV6,11,18-DNA positive rate respectively(P＜0.001),and the different HPV-DNA was simultaneously tested in one cerv ical carcinoma; last, not all cases of P53 mutations had p53 proteins posi tive, but the cases of P53 mutations and p53 proteins negative certainly had HPV infections, and HPV positive cases were much more than its negative one in the cases of p53 proteins positive(P＜0.001). These results proved that the oncogenesis of cervical carcinoma is mainly associated with HPV16 infections, and second related to P53 (exon7～8) mutations. p53 proteins positive results from P53 mutations or/and HPV infections in cervical carc inoma.
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0.1).Not all cases of p53 mutations were with p53 protein positive.Conclusion:p53 (exon 7 8) mutation is not a main factor for the structural abnormality of p53 protein in the oncogenesis of cervical cancer.